THE™ His Tag Antibody, mAb, Mouse：THE™ His Tag Antibody, mAb, Mouse recognizes His tags localized at the C-terminal, N-terminal, and internal region of fusion proteins.

產品型號：A00186

更新時間：2025-11-06

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產品介紹

產品概述

Specificity	THE™ His Tag Antibody, mAb, Mouse recognizes His tags localized at the C-terminal, N-terminal, and internal region of fusion proteins.
Host Species	Mouse
Immunogen	A synthetic peptide HHHHHH coupled to KLH
Conjugate	Unconjugated

應用指導

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
ELISA	0.05-0.2 µg/ml
Western Blot	0.1-0.2 µg/ml
Immunoprecipitation (IP)	1 µg/ml
Immunocytochemistry/Immunofluorescence (ICC/IF)	1 µg/ml
Flow Cytometry	1 µg/ml
Time-resolved fluorescence energy transfer (TR-FRET)	User-optimized

產品屬性

Form	Lyophilized
Storage Buffer	Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution	Reconstitute the lyophilized powder with sterile water (or equivalent) to a final concentration of 0.5 mg/mL.
Storage Instructions	The product remains stable for 1 year at -20°C from the date of receipt. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.Avoid repeated freezing and thawing cycles.
Purification	Purified by Protein A affinity chromatography.
Isotype	Mouse IgG1,κ
Clonality	Monoclonal
Clone ID	6G2A9
Note	GenScript can offer this product according to your requirement, including product size, buffer components, etc.