Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo, PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, human skeletal development, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets.

產(chǎn)品簡介

 Species： Rat

 Protein Construction ：Expressed with an N-terminal Met. PDGF-BB (Ser74-Thr182) Accession # Q05028 

Purity： > 95% as analyzed by SDS-PAGE 

Endotoxin Level ：< 0.2 EU/μg of protein by gel clotting method 

Biological Activity： ED50 < 2.0 ng/ml, measured by a cell proliferation assay using 3T3 Cells, corresponding to a specific activity of > 5.0 × 105 units/mg. Expression System： E. coli

Apparent Molecular Weight ：~24.6 kDa, on SDS-PAGE under non-reducing conditions.

 Formulation ：Lyophilized after extensive dialysis against 20 mM acetic acid. 

Reconstitution： It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in 20 mM acetic acid up to 100 μg/ml. 

Storage & Stability ：Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

技術(shù)背景

 Target Background ：Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo, PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, human skeletal development, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets. 

Synonyms： PDGFBB; IBGC5; PDGF-2; PDGF2; SIS; SSV; c-sis; platelet derived growth factor subunit B

 For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.